400m Hurdles is another version of 400m and 110m Hurdles. It is the longer version. It is played by using the Touch Screen for the DS and the Wii Remote for the Wii.
- Sonic: Perform 7 Sprint Jumps
- Daisy: Don't knock a hurdle down
- Yoshi: Beat Tails on his Top Speed
- Waluigi: Overcome a 3 second handicap and finish 1st
- Bowser: Finish 1st between 45 and 53 seconds without knocking a hurdle down.
- Daisy: Finish between 45 and 53 seconds without knocking a hurdle down.
- Wario: Beat Luigi on his Top Speed
- Sonic: Finish 1st while Sprinting